Browsing by Author "Ziediņa, Ieva"

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    High-Risk Human Papillomavirus Infection in Latvian Male Kidney Transplant Recipients
    (2016-08-01) Čistjakovs, Maksims; Sultanova, Alina; Jermakova, Olga; Čapenko, Svetlana; Lesiņa-Korne, Baiba; Rozentāls, Rafails; Murovska, Modra; Ziediņa, Ieva; Institute of Microbiology and Virology; Department of Internal Diseases
    Kidney transplant recipients have higher incidence of human papillomavirus (HPV)-related malignancies, but studies on the natural history of HPV infection are insufficient, especially regarding in male recipients. The aim of this study was to evaluate the course of high-risk HPV (HR-HPV) infection after kidney allograft transplantation in male recipients: to estimate frequency and activity of HR-HPV infection under immune system suppression. Twenty male renal recipients (age 20-68) were enrolled in this investigation and examined in dynamics. Peripheral EDTA-blood samples and urine samples were collected from each patient 2 weeks, 6 months and 12 months after transplantation. Polymerase chain reaction (PCR) with consensus primers was used for initial detection of high range HPV types, a commercial qPCR kit for detection of HR-HPV load in urine samples and ELISA for detection of serum IgG class antibodies to HR-HPV L1-capsid protein. Overall, combining molecular (HR-HPV genomic sequences detected by real-time PCR) and serological studies (IgG class antibodies to HR-HPV L1-capsids' protein), high frequency of HRHPV infection among male kidney transplant recipients (9/20; 45%) was showed. However, the majority of HR-HPV positive recipients (7/9; 78%) showed signs of infection clearance. It means that, despite the applied immune suppressive therapy, the host's immune system is capable of dealing with HR-HPV infection up to the 12th month after transplantation. However, the sample size should be increased to enable through statistical analysis before final conclusions are made.
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    Leucine-Rich Alpha-2-Glycoprotein (LRG-1) as a Potential Kidney Injury Marker in Kidney Transplant Recipients
    (2022-09) Popova, Anna; Vasiļvolfa, Aiga; Rācenis, Kārlis; Erts, Renārs; Šlisere, Baiba; Saulīte, Anna Jana; Ziediņa, Ieva; Folkmane, Inese; Čerņevskis, Harijs; Kroiča, Juta; Pētersons, Aivars; Kuzema, Viktorija; Department of Biology and Microbiology; Department of Internal Diseases
    Background: Material/Methods: Results: Conclusions: Kidney transplantation is the treatment of choice for most patients with end-stage renal disease. To improve patient and transplant survival, non-invasive diagnostic methods for different pathologies are important. Leucine-rich alpha-2-glycoprotein (LRG-1) is an innovative biomarker that is elevated in cases of angiogenesis, inflammation, and kidney injury. However, there are limited data about the diagnostic role of LRG-1 in kidney transplant recipients. The aim of this study was to evaluate the association between serum LRG-1, urine LRG-1, and kidney transplant function and injury. We enrolled 35 kidney transplant recipients in the study. LRG-1 in the serum and urine was detected using ELISA. We evaluated the correlation of serum and urine LRG-1 with traditional serum and urine kidney injury markers. A higher level of serum LRG-1 correlates with a higher level of urine LRG-1. Serum LRG-1 has a positive correlation with transplant age, serum urea, serum creatinine, serum cystatin C, proteinuria, and fractional excretion of sodium (FENa) and a negative correlation with hemoglobin and estimated glomerular filtration rate (eGFR). Urine LRG-1 has a positive correlation with serum cystatin C, proteinuria, and urine neutrophil gelatinase-as-sociated lipocalin (NGAL). Higher levels of serum and urine LRG-1 are associated with kidney transplant injury and functional deterioration. Thus, LRG-1 might be also as a biomarker for tubular dysfunction in patients after kidney transplantation.
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    Poliomas BK vīrusa infekcija pacientiem ar nieres transplantātu. Promocijas darba kopsavilkums
    (Rīgas Stradiņa universitāte, 2011) Ziediņa, Ieva; Rozentāls, Rafails; Čapenko, Svetlana
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    Poliomas BK vīrusa infekcija pacientiem ar nieres transplantātu. Promocijas darbs
    (Rīgas Stradiņa universitāte, 2011) Ziediņa, Ieva; Rozentāls, Rafails; Čapenko, Svetlana
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    Polyoma BK Virus Infection in Renal Transplant Recipients. Summary of the Doctoral Thesis
    (Rīga Stradiņš University, 2011) Ziediņa, Ieva; Rozentāls, Rafails; Čapenko, Svetlana
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    SERUM AND URINE LEUCINE RICH ALPHA-2-GLYCOPROTEIN-1 IS ASSOCIATED WITH KIDNEY TRANSPLANT INJURY AND FAILURE
    (2021-05) Vasiļvolfa, Aiga; Kroiča, Juta; Popova, Anna; Rācenis, Kārlis; Šlisere, Baiba; Ziediņa, Ieva; Folkmane, Inese; Lejnieks, Aivars; Čerņevskis, Harijs; Pētersons, Aivars; Kuzema, Viktorija; Rīga Stradiņš University