Browsing by Author "Vilde, Aija"

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    Hand-Washing Video Dataset Annotated According to the World Health Organization’s Hand-Washing Guidelines
    (2021-04-07) Lulla, Martins; Rutkovskis, Aleksejs; Slavinska, Andreta; Vilde, Aija; Gromova, Anastasija; Ivanovs, Maksims; Skadins, Ansis; Kadikis, Roberts; Elsts, Atis; Medical Education Technology Centre
    Washing hands is one of the most important ways to prevent infectious diseases, including COVID-19. The World Health Organization (WHO) has published hand-washing guidelines. This paper presents a large real-world dataset with videos recording medical staff washing their hands as part of their normal job duties in the Pauls Stradins Clinical University Hospital. There are 3185 hand-washing episodes in total, each of which is annotated by up to seven different persons. The annotations classify the washing movements according to the WHO guidelines by marking each frame in each video with a certain movement code. The intention of this “in-the-wild” dataset is two-fold: to serve as a basis for training machine-learning classifiers for automated hand-washing movement recognition and quality control, and to allow to investigation of the real-world quality of washing performed by working medical staff. We demonstrate how the data can be used to train a machine-learning classifier that achieves classification accuracy of 0.7511 on a test dataset.
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    Successful Bacteriophage-Antibiotic Combination Therapy against Multidrug-Resistant Pseudomonas aeruginosa Left Ventricular Assist Device Driveline Infection
    (2023-05) Racenis, Karlis; Lacis, Janis; Rezevska, Dace; Mukane, Laima; Vilde, Aija; Putnins, Ints; Djebara, Sarah; Merabishvili, Maya; Pirnay, Jean Paul; Kalnina, Marika; Petersons, Aivars; Stradins, Peteris; Maurins, Sandis; Kroica, Juta; Department of Biology and Microbiology; Department of Internal Diseases; Department of Surgery
    There is considerable interest in the use of bacteriophages (phages) to treat Pseudomonas aeruginosa infections associated with left ventricular assist devices (LVADs). These infections are often challenging to manage due to high rates of multidrug resistance and biofilm formation, which could potentially be overcome with the use of phages. We report a case of a 54-year-old man with relapsing multidrug-resistant P. aeruginosa LVAD driveline infection, who was treated with a combination of two lytic antipseudomonal phages administered intravenously and locally. Treatment was combined with LVAD driveline repositioning and systemic antibiotic administration, resulting in a successful outcome with clinical cure and eradication of the targeted bacteria. However, laboratory in vitro models showed that phages alone could not eradicate biofilms but could prevent biofilm formation. Phage-resistant bacterial strains evolved in biofilm models and showed decreased susceptibility to the phages used. Further studies are needed to understand the complexity of phage resistance and the interaction of phages and antibiotics. Our results indicate that the combination of phages, antibiotics, and surgical intervention can have great potential in treating LVAD-associated infections. More than 21 months post-treatment, our patient remains cured of the infection.