Browsing by Author "Skudrins, Gundars"

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    Exploring the Potential of Supercritical Fluid Extraction of Matricaria chamomilla White Ray Florets as a Source of Bioactive (Cosmetic) Ingredients
    (2023-05-12) Pastare, Laura; Berga, Marta; Kienkas, Liene; Boroduskis, Martins; Ramata-Stunda, Anna; Reihmane, Dace; Senkovs, Maris; Skudrins, Gundars; Nakurte, Ilva
    Aromatic and medicinal plants are a great source of useful bioactive compounds for use in cosmetics, drugs, and dietary supplements. This study investigated the potential of using supercritical fluid extracts obtained from Matricaria chamomilla white ray florets, a kind of industrial herbal byproduct, as a source of bioactive cosmetic ingredients. Response surface methodology to optimize the supercritical fluid extraction process by analyzing the impact of pressure and temperature on yield and the main bioactive compound groups were used. High-throughput 96-well plate spectrophotometric methods were used to analyze the extracts for total phenols, flavonoids, tannins, and sugars, as well as their antioxidant capacity. Gas chromatography and liquid chromatography-mass spectrometry was used to determine the phytochemical composition of the extracts. The extracts were also analyzed for antimicrobial activity, cytotoxicity, phototoxicity, and melanin content. Statistical analysis was performed to establish correlations between the extracts and develop models to predict the targeted phytochemical recovery and chemical and biological activities. The results show that the extracts contained a diverse range of phytochemical classes and had cytotoxic, proliferation-reducing, and antimicrobial activities, making them potentially useful in cosmetic formulations. This study provides valuable insights for further research on the uses and mechanisms of action of these extracts.
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    In Vitro Safety and Efficacy Evaluation of a Juniperus communis Callus Culture Extract and Matricaria recutita Processing Waste Extract Combination as a Cosmetic Ingredient
    (2024-01-18) Ramata-Stunda, Anna; Boroduskis, Martins; Pastare, Laura; Berga, Marta; Kienkas, Liene; Patetko, Liene; Skudrins, Gundars; Reihmane, Dace; Nakurte, Ilva
    For skin health promotion and cosmetic applications, combinations of plant cell extracts are extensively utilized. As most natural ingredient suppliers offer crude extracts from individual plants or specific isolated compounds, the potential interactions between them are assessed in the development phase of cosmetic products. The industry seeks extract combinations that have undergone optimization and scrutiny for their bioactivities. This study presents a combination of two sustainably produced botanical ingredients and outlines their chemical composition, in vitro safety, and bioactivity for skin health enhancement. The amalgamation comprises the extract of Matricaria recutita processing waste and the extract from Juniperus communis callus culture. Chemical analysis revealed distinct compounds within the extracts, and their combination led to a broader array of potentially synergistic compounds. In vitro assessments on skin cells demonstrated that the combination possesses robust antioxidant properties and the ability to stimulate keratinocyte proliferation, along with regulating collagen type I and matrix metalloproteinase 1 (MMP-1) production by dermal fibroblasts. The identified traits of this combination render it an appealing cosmetic component. To the best of our knowledge, this represents the first case when the extracts derived from medicinal plant processing waste and biotechnological plant cell cultivation processes have been combined and evaluated for their bioactivity.
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    Phenolic Content and Anti-Inflammatory Activity of Cultivated and Wild-Type Galium odoratum Extracts in Murine Bone Marrow-Derived Macrophages
    (2024-11-25) Razživina, Valērija; Vasiljeva, Anna; Kronberga, Arta; Skudrins, Gundars; Nakurte, Ilva; Žogota, Marta; Mezaka, Ieva; Pugovics, Osvalds; Grinberga, Solveiga; Dambrova, Maija; Sīle, Inga; Department of Pharmaceutical Chemistry; Laboratory of Finished Dosage Forms; Department of Applied Pharmacy
    Galium odoratum, commonly known as sweet woodruff, is a perennial herbaceous plant that contains coumarin and is recognized for its medicinal properties. In this study, the influence of sunlight exposure on the phytochemical composition and anti-inflammatory potential of G. odoratum extracts is assessed. The extracts from cultivated and wild-grown plants were analyzed via chromatographic and mass spectrometric methods. In addition, the total phenolic content, antioxidant activity, and effects on macrophage polarization were assessed. The results revealed that while coumarin levels remain stable regardless of environmental conditions, phenolic content and antioxidant activity increase significantly under sun-grown conditions, with chlorogenic acid and rutin identified as major contributing compounds. Additionally, the extracts exhibited anti-inflammatory activity, effectively reducing the M1 macrophage population involved in inflammatory responses. These findings suggest that controlled sunlight exposure can enhance the bioactive profile of G. odoratum. This research highlights the critical role of environmental management in optimizing the medicinal properties of G. odoratum, providing a foundation for its future use in natural therapeutic applications.