Browsing by Author "Mironov, Vladimir A."

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    Commercial articulated collaborative in situ 3D bioprinter for skin wound healing
    (2023-01-31) Levin, Aleksandr A; Karalkin, Pavel A.; Koudan, Elizaveta V.; Senatov, Fedor S.; Parfenov, Vladislav A.; Lvov, Vladislav; Petrov, Stanislav; Pereira, Frederiko D.A.S.; Kovalev, Alexey V.; Osidak, Egor; Domogatsky, Sergey P.; Manturova, Natalya E.; Kasyanov, Vladimir; Sergeeva, Natalia S.; Zorin, Vadim L.; Khesuani, Yusef D.; Mironov, Vladimir A.; Joint Laboratory of Traumatology and Orthopaedics
    In situ bioprinting is one of the most clinically relevant techniques in the emerging bioprinting technology because it could be performed directly on the human body in the operating room and it does not require bioreactors for post-printing tissue maturation. However, commercial in situ bioprinters are still not available on the market. In this study, we demonstrated the benefit of the originally developed first commercial articulated collaborative in situ bioprinter for the treatment of full-thickness wounds in rat and porcine models. We used an articulated and collaborative robotic arm from company KUKA and developed original printhead and correspondence software enabling in situ bioprinting on curve and moving surfaces. The results of in vitro and in vivo experiments show that in situ bioprinting of bioink induces a strong hydrogel adhesion and enables printing on curved surfaces of wet tissues with a high level of fidelity. The in situ bioprinter was convenient to use in the operating room. Additional in vitro experiments (in vitro collagen contraction assay and in vitro 3D angiogenesis assay) and histological analyses demonstrated that in situ bioprinting improves the quality of wound healing in rat and porcine skin wounds.
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    Design, Fabrication, and Application of Mini-Scaffolds for Cell Components in Tissue Engineering
    (2022-12) Mironov, Vladimir A.; Senatov, Fedor S.; Koudan, Elizaveta V.; Pereira, Frederico D.A.S.; Kasyanov, Vladimir A.; Granjeiro, Jose Mauro; Baptista, Leandra Santos; Joint Laboratory of Traumatology and Orthopaedics
    The concept of “lockyballs” or interlockable mini-scaffolds fabricated by two-photon polymerization from biodegradable polymers for the encagement of tissue spheroids and their delivery into the desired location in the human body has been recently introduced. In order to improve control of delivery, positioning, and assembly of mini-scaffolds with tissue spheroids inside, they must be functionalized. This review describes the design, fabrication, and functionalization of mini-scaffolds as well as perspectives on their application in tissue engineering for precisely controlled cell and mini-tissue delivery and patterning. The development of functionalized mini-scaffolds advances the original concept of “lockyballs” and opens exciting new prospectives for mini-scaffolds’ applications in tissue engineering and regenerative medicine and their eventual clinical translation.