Browsing by Author "Mezaka, Ieva"

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    The Cultivation of Chelidonium majus L. Increased the Total Alkaloid Content and Cytotoxic Activity Compared with Those of Wild-Grown Plants
    (2021-09) Krizhanovska, Valerija; Sile, Inga; Kronberga, Arta; Nakurte, Ilva; Mezaka, Ieva; Dambrova, Maija; Pugovics, Osvalds; Grinberga, Solveiga; Department of Applied Pharmacy; Department of Pharmaceutical Chemistry
    The effect of cultivation practises on both the phytochemical profile and biological activity of aqueous ethanol extracts of Chelidonium majus L. was studied. Extracts were prepared from aerial parts of the same plant population collected in the wild and grown under organic farming conditions. Both qualitative and quantitative analyses of alkaloids and flavonoid derivatives were performed by LC/MS methods, and the cytotoxicity of lyophilised extracts was studied in B16-F10, HepG2, and CaCo-2 cells. Coptisine was the dominant alkaloid of extracts prepared from wild-grown plants, whereas after cultivation, chelidonine was the most abundant alkaloid. The total alkaloid content was significantly increased by cultivation. Ten flavonol glycoconjugates were identified in C. majus extracts, and quantitative analysis did not reveal significant differences between extracts prepared from wild-grown and cultivated specimens. Treatment with C. majus extracts resulted in a dose-dependent increase in cytotoxicity in all three cell lines. The extracts prepared from cultivated specimens showed higher cytotoxicity than the extracts prepared from wild-grown plants. The strongest cytotoxic effect of cultivated C. majus was observed in B16-F10 cells (IC50 = 174.98 ± 1.12 µg/mL). Cultivation-induced differences in the phytochemical composition of C. majus extracts resulted in significant increases in the cytotoxic activities of the preparations.
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    Phenolic Content and Anti-Inflammatory Activity of Cultivated and Wild-Type Galium odoratum Extracts in Murine Bone Marrow-Derived Macrophages
    (2024-11-25) Razživina, Valērija; Vasiljeva, Anna; Kronberga, Arta; Skudrins, Gundars; Nakurte, Ilva; Žogota, Marta; Mezaka, Ieva; Pugovics, Osvalds; Grinberga, Solveiga; Dambrova, Maija; Sīle, Inga; Department of Pharmaceutical Chemistry; Laboratory of Finished Dosage Forms; Department of Applied Pharmacy
    Galium odoratum, commonly known as sweet woodruff, is a perennial herbaceous plant that contains coumarin and is recognized for its medicinal properties. In this study, the influence of sunlight exposure on the phytochemical composition and anti-inflammatory potential of G. odoratum extracts is assessed. The extracts from cultivated and wild-grown plants were analyzed via chromatographic and mass spectrometric methods. In addition, the total phenolic content, antioxidant activity, and effects on macrophage polarization were assessed. The results revealed that while coumarin levels remain stable regardless of environmental conditions, phenolic content and antioxidant activity increase significantly under sun-grown conditions, with chlorogenic acid and rutin identified as major contributing compounds. Additionally, the extracts exhibited anti-inflammatory activity, effectively reducing the M1 macrophage population involved in inflammatory responses. These findings suggest that controlled sunlight exposure can enhance the bioactive profile of G. odoratum. This research highlights the critical role of environmental management in optimizing the medicinal properties of G. odoratum, providing a foundation for its future use in natural therapeutic applications.
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    Wild-Grown and Cultivated Glechoma hederacea L. : Chemical Composition and Potential for Cultivation in Organic Farming Conditions
    (2022-03-01) Sile, Inga; Krizhanovska, Valerija; Nakurte, Ilva; Mezaka, Ieva; Kalane, Laura; Filipovs, Jevgenijs; Vecvanags, Alekss; Pugovics, Osvalds; Grinberga, Solveiga; Dambrova, Maija; Kronberga, Arta; Department of Applied Pharmacy; Department of Pharmaceutical Chemistry
    Glechoma hederacea L. is a medicinal plant that is known in traditional medicine for its anti-inflammatory, antibacterial, antiviral, and anticancer properties. This study evaluated the potential for commercial production of G. hederacea and compared the chemical composition and activity of 70% ethanol extracts and steam-distilled essential oils from wild-grown and cultivated G. hederacea collected in different harvesting periods. The main compounds identified in the 70% ethanol extracts were phenolic acids (chlorogenic and rosmarinic acids) and flavonoid O-glycosides. The essential oil varied in the three accessions in the range of 0.32-2.98 mL/kg -1 of dry weight. The extracts possessed potent antioxidant and anti-inflammatory properties in LPS-treated bone-marrow-derived macrophages. The results of flow cytometry show that extracts from different vegetation periods reduced the conversion of macrophages to the proinflammatory phenotype M1. The chemical composition varied the most with the different harvesting periods, and the most suitable periods were the flowering and vegetative phases for the polyphenolic compounds and essential oils, respectively. G. hederacea can be successfully grown under organic farming conditions, and cultivation does not significantly affect the chemical composition and biological activity compared to wild-grown plants.