Browsing by Author "Karalkin, Pavel A."

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    Commercial articulated collaborative in situ 3D bioprinter for skin wound healing
    (2023-01-31) Levin, Aleksandr A; Karalkin, Pavel A.; Koudan, Elizaveta V.; Senatov, Fedor S.; Parfenov, Vladislav A.; Lvov, Vladislav; Petrov, Stanislav; Pereira, Frederiko D.A.S.; Kovalev, Alexey V.; Osidak, Egor; Domogatsky, Sergey P.; Manturova, Natalya E.; Kasyanov, Vladimir; Sergeeva, Natalia S.; Zorin, Vadim L.; Khesuani, Yusef D.; Mironov, Vladimir A.; Joint Laboratory of Traumatology and Orthopaedics
    In situ bioprinting is one of the most clinically relevant techniques in the emerging bioprinting technology because it could be performed directly on the human body in the operating room and it does not require bioreactors for post-printing tissue maturation. However, commercial in situ bioprinters are still not available on the market. In this study, we demonstrated the benefit of the originally developed first commercial articulated collaborative in situ bioprinter for the treatment of full-thickness wounds in rat and porcine models. We used an articulated and collaborative robotic arm from company KUKA and developed original printhead and correspondence software enabling in situ bioprinting on curve and moving surfaces. The results of in vitro and in vivo experiments show that in situ bioprinting of bioink induces a strong hydrogel adhesion and enables printing on curved surfaces of wet tissues with a high level of fidelity. The in situ bioprinter was convenient to use in the operating room. Additional in vitro experiments (in vitro collagen contraction assay and in vitro 3D angiogenesis assay) and histological analyses demonstrated that in situ bioprinting improves the quality of wound healing in rat and porcine skin wounds.