Browsing by Author "Gatina, Linda"

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    The amount of ghrelin-immunoreactive cells in the abomasum and intestines of 13-14-week-old calves supplemented with Jerusalem artichoke flour alone or in combination with Saccharomyces cerevisiae yeast
    (2022-04-26) Jonova, Sintija; Ilgaza, Aija; Ilgazs, Agris; Zolovs, Maksims; Gatina, Linda; Statistics Unit
    Background and Aim: The use of antibiotics in animals for disease prevention and productivity has been banned in the European Union since 2006. Possible alternatives can be used prebiotics, probiotics, and synbiotics. These compounds can improve feed digestion and absorption in the gastrointestinal tract with identical nutrient uptake, while imparting the feeling of satiety, which reduces the activity of ghrelin-immunoreactive (IR) cells. The number of studies performed on the activity of ghrelin-IR cells in ruminants is insufficient. In particular, there are few such studies in calves during the transition period from being a relatively monogastric animal to a ruminant. The present study aimed to evaluate the effect of Jerusalem artichoke flour (containing ∼50% prebiotic inulin) and a new, commercially unavailable synbiotic (combination of Jerusalem artichoke flour and Saccharomyces cerevisiae strain 1026) on the amount of ghrelin-IR cells in the abomasum and intestines of 13-14-week-old calves. Materials and Methods: Fifteen crossbreed, Holstein Friesian and Red Holstein calves (Bos taurus) (32±4 days, 72.1±11.34 kg) were used. Calves were allocated into three groups: Control group (CoG, n=5) received the standard diet, prebiotic group (PreG, n=5) received 12 g of flour of Jerusalem artichoke (Helianthus tuberosus) per head containing 6 g of prebiotic inulin in addition to the standard diet, and synbiotic group (SynG, n=5) received a synbiotic in addition to the standard diet which consisted of two different products: 12 g of flour of Jerusalem artichoke per head containing 6 g of prebiotic inulin and probiotic 5 g of a yeast S. cerevisiae strain 1026. Feed additives were added to the concentrate once a day for 56 days. On days 1, 28, and 56, the live weight of the calves was determined. On day 56 of the experiment, three calves from each group were slaughtered. Histological samples were collected from the two parts of each calf abomasum: Pars pylorica and pars fundalis and the middle part of the duodenum and jejunum. Immunohistochemical tissue staining methods were used to detect ghrelin-IR cells. Results: The live weight of the slaughtered calves on day 56 was 115.3±21.73 kg in CoG, 130.0±17.32 kg in PreG, and 119.0±7.94 kg in SynG. Ghrelin-IR cells were more abundantly localized in the cytoplasm of the abomasum muscle gland cells in pars fundalis and pars pylorica, and to a lesser extent in the duodenum and jejunum. The number of ghrelin-IR cells in the abomasal fundic gland area was significantly higher in the CoG, than in the PreG and SynG (p=0.0001), while the difference between the PreG and SynG was not significant (p=0.700). Conclusion: The addition of Jerusalem artichoke flour and its combination with the yeast S.cerevisiae stain 1026 in calves resulted in a lower number of ghrelin-IR cells in the abomasum, duodenum, and jejunum and, although insignificantly, increased live weight (p=0.491), suggesting that calves in these groups with the same feed intake as the CoG had a better breakdown of nutrients, thus having a longer feeling of satiety.
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    Tissue-Welding Device : Considerable Advantages for Spleen Surgery Based on Histological and Cardiorespiratory Investigation
    (2022-09-24) Gatina, Linda; Pigiņka-Vjačeslavova, Inga; Bērziņa, Dace; Zolovs, Maksims; Statistics Unit
    During splenic surgery, it is important to control blood loss and the potential risk of cardiac arrhythmia. The best way to prevent complications from surgery is to use the appropriate surgical devices; however, there is no guideline for the use of specific surgical devices for spleen incision. Therefore, the aim of this research was to compare the interactions of various surgical devices with spleen tissue, their cardiorespiratory effects during incision, and subsequent spleen surgical wound healing. A total of 75 rabbits were included in the study. CO2 laser (n = 15), radiofrequency device (n = 15), electrocoagulator (n = 15), tissue-welding device (n = 15), and scalpel (n = 15) were used to make incisions in rabbits' spleens. Spleen biopsies of the incision area were taken from each animal at the day 0, 7, and 14 after surgery. Contactless thermography was performed during surgery. Suturing was not used after incision with the tissue-welding device, but incisions made by other surgical devices were sutured. The results showed that the width of spleen necrosis differed significantly between the various surgical devices used on spleen tissues. There was a positive, strong, and linear association between necrosis width and the tissue temperature of cutting edges. Significant increases in the heart rate were observed during spleen surgery performed with laser, scalpel, and radiofrequency devices. In conclusion, the tissue-welding device confers a significant advantage in spleen surgery, as there is neither a need for sutures nor a significant deviation in the heart rate.