Browsing by Author "Folkmane, Inese"

Now showing 1 - 7 of 7
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    Anemia as a complication of parvovirus B19 infection : In renal transplant recipients
    (2012) Čapenko, Svetlana; Kozireva, Svetlana; Folkmane, Inese; Bernarde, Kristina; Rozentals, Rafails; Murovska, Modra; Institute of Microbiology and Virology
    Background: The frequency of B19 infection in renal transplant donors and recipients was studied to determine the significance of active viral infection in the development of anemia. Material and Methods. Serum, plasma, and peripheral blood leukocyte samples of 47 renal transplant donors, 38 recipients with anemia (Group 1), and 25 without anemia (Group 2) after renal transplantation were evaluated for the presence of anti-B19 specific antibodies (ELISA) and B19 DNA (nPCR). Results: Active persistent B19 infection after renal transplantation was detected in 12 of the 38 in the Group 1 (10 had reactivation and 2 primary infection), and none of the recipients in the Group 2 had it. Of the 12 recipients in the Group 1, 10 were seropositive and 2 seronegative before renal transplantation; 10 received the transplants from the seropositive and 2 from seronegative donors. rHuEPO therapy-resistant severe anemia was detected only in the recipients with active B19 infection after renal transplantation in the Group 1 (7/12). The logistic regression analysis revealed a significant relationship between active B19 infection and severe anemia (OR, 0.039; 95% CI, 0.006-0.257; P=0.001). Conclusions: Active B19 infection was documented only in the anemic recipients and could be associated with the development of severe anemia after renal transplantation. This allows us to recommend concurrent screening for viral DNA in plasma and detection of anti-B19 IgM class antibodies. To find the association between B19 infection and the development of anemia, further investigations are necessary.
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    Assessment of HHV-6 and HHV-7 in patients after kidney transplantation
    (2013) Folkmane, Inese; Čapenko, Svetlana; Adamsone, Inara; Folkmane, Elizabete; Murovska, Modra; Institute of Microbiology and Virology
    Human herpesviruses HHV-6 and HHV-7 reactivation in transplantation is associated with indirect immunomodulatory effects, such as cytomegalovirus (CMV) disease, increased opportunistic infections, graft dysfunction and acute rejection (AR). In this study, we analysed the clinical and immunological outcomes in renal transplant recipients (RTR) with active HHV-6 and HHV-7 infection. Between January 2007 and December 2007, clinical, virological and immunological tests were carried out in 46 RTR. The patients were divided into three groups: with active HHV-6 infection; with active HHV-7 infection; and without infection (control). The mean follow-up was 14 ± 2.5 months. At three months after renal transplantation (RT), active CMV infection was present in 12 (26%); HHV-6 in four (8.6%); and HHV-7 in nine (19.5%) of RTR. Active ß-herpesviruses infection was not associated with more frequent AR and worsening of graft function in recipients at different times after RT. The lymphocyte subsets (CD3+; CD4+ and CD8+ cell count) were considerably lower in RTR before RT. At 3 months after RT CD19+ and CD25+ cell counts were significantly increased in the HHV-7 group compared with the control group (P < 0.05). Significant differences were not found in clinical and immunological outcomes between patients with active ß-herpesviruses infection and those without active ß-herpesviruses infection.
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    CD63 and Dna Mismatch Repair Protein Expression in Prostate Cancer
    (2021-06-01) Folkmanis, Kristofs; Eglītis, Jānis; Jakubovskis, Māris; Lietuvietis, Vilnis; Folkmane, Inese; Isajevs, Sergejs; Rīga Stradiņš University
    Protein expression levels in immunohistochemistry and molecular biomarkers have been reported for their ability to predict recurrence, progression, development of metastases, and patient survival. The molecular features in low- and high-grade prostate cancer can differ and influence treatment decision and prognosis. The objective of the current study was to compare the expression of exosomal biomarkers CD63 and mismatch repair proteins (MSH2, MSH6, MLH1, and PMS2) by immunohistochemistry (IHC) in tissue of patients with prostate cancer and benign hyperplasia. Altogether, 62 patients with prostate acinar adenocarcinoma and 20 patients with prostate benign hyperplasia were enrolled in this retrospective study. CD63, MSH2, MSH6, MLH1, and PMS2 expression was analysed by immunohistochemistry. The obtained results showed that CD63 expression was significantly higher in patients with Grade III-V prostate cancer compared to Grade I-II, respectively; 2.23 (1-3) vs 0.92 (0-2) score, p = 0.001. In addition, a significant positive correlation between CD63 expression and grade groups was revealed (Rho = +0.54; p < 0.0001). Furthermore, progression-free survival was significantly higher in patients with low CD63 expression, compared to high CD63 expression (p = 0.0007). MMR expression was absent in 14 patients (four patients with Grade I-II cancer and 10 patients with Grade III-cancer). MMR was present in all cases of benign prostate hyperplasia (mild to moderate staining). The conclusion was that high grade prostate cancer (Grade groups III-V) was characterised by increased CD63 expression, which correlated with progression-free survival.
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    Clinicopathological Significance of Exosomal Proteins CD9 and CD63 and DNA Mismatch Repair Proteins in Prostate Adenocarcinoma and Benign Hyperplasia
    (2022-02) Folkmanis, Kristofs; Junk, Elizabete; Merdane, Evelina; Folkmane, Inese; Folkmanis, Valdis; Ivanovs, Igors; Eglitis, Janis; Jakubovskis, Maris; Laabs, Sven; Isajevs, Sergejs; Lietuvietis, Vilnis; Faculty of Medicine
    Introduction. Recently, it has been shown that exosomal biomarkers and DNA mismatch repair proteins (MMR) could play an important role in cancer risk stratification and prognosis assessment. The gold standard for prostate carcinoma (PCa) diagnosis is biopsy and histopathological examination. Thus, the complex evaluation of exosomal and MMR proteins could be beneficial for prostate cancer risk stratification and diagnostics. The aim of the current study was to evaluate and compare the expression of exosomal proteins CD9 and CD63 and MMR proteins in the tissue of patients with prostate benign hyperplasia (BPH) and PCa. Methods. The study was retrospective. Altogether, 92 patients with PCa and 20 patients with BPH (control group) were enrolled in the study. Exosomal and MMR protein expression was analyzed by immunohistochemistry (IHC). The follow-up for each PCa patient in our study lasted till disease progression and/or a maximum of 5 years. Results. Low-grade PCa was observed in 56 patients and high-grade PCa in 36 patients. CD63 expression was significantly higher in patients with high-grade PCa compared to those with low-grade PCa. CD9 expression was significantly downregulated in PCa patients compared to the control group. MMR protein expression deficiency was observed in 10 PCa patients. MMR proteins were maintained in all cases of BPH. The study found a negative correlation between MMR protein loss and PCa ISUP grade groups. Progression-free survival (PFS) in patients with MMR deficiency was significantly shorter than in patients with maintained MMR expression. Conclusions. CD9 protein expression was downregulated in PCa, compared to BPH, while CD63 protein expression was upregulated in high-grade PCa but downregulated in low-grade PCa. CD63 protein upregulation, CD9 downregulation, and loss of MMR protein characterized the shorter PFS of high-grade PCa patients. CD9, CD63, and MMR could be the routine immunohistochemical biomarkers for the diagnosis and risk stratification of PCa.
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    Leucine-Rich Alpha-2-Glycoprotein (LRG-1) as a Potential Kidney Injury Marker in Kidney Transplant Recipients
    (2022-09) Popova, Anna; Vasiļvolfa, Aiga; Rācenis, Kārlis; Erts, Renārs; Šlisere, Baiba; Saulīte, Anna Jana; Ziediņa, Ieva; Folkmane, Inese; Čerņevskis, Harijs; Kroiča, Juta; Pētersons, Aivars; Kuzema, Viktorija; Department of Biology and Microbiology; Department of Internal Diseases
    Background: Material/Methods: Results: Conclusions: Kidney transplantation is the treatment of choice for most patients with end-stage renal disease. To improve patient and transplant survival, non-invasive diagnostic methods for different pathologies are important. Leucine-rich alpha-2-glycoprotein (LRG-1) is an innovative biomarker that is elevated in cases of angiogenesis, inflammation, and kidney injury. However, there are limited data about the diagnostic role of LRG-1 in kidney transplant recipients. The aim of this study was to evaluate the association between serum LRG-1, urine LRG-1, and kidney transplant function and injury. We enrolled 35 kidney transplant recipients in the study. LRG-1 in the serum and urine was detected using ELISA. We evaluated the correlation of serum and urine LRG-1 with traditional serum and urine kidney injury markers. A higher level of serum LRG-1 correlates with a higher level of urine LRG-1. Serum LRG-1 has a positive correlation with transplant age, serum urea, serum creatinine, serum cystatin C, proteinuria, and fractional excretion of sodium (FENa) and a negative correlation with hemoglobin and estimated glomerular filtration rate (eGFR). Urine LRG-1 has a positive correlation with serum cystatin C, proteinuria, and urine neutrophil gelatinase-as-sociated lipocalin (NGAL). Higher levels of serum and urine LRG-1 are associated with kidney transplant injury and functional deterioration. Thus, LRG-1 might be also as a biomarker for tubular dysfunction in patients after kidney transplantation.
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    SERUM AND URINE LEUCINE RICH ALPHA-2-GLYCOPROTEIN-1 IS ASSOCIATED WITH KIDNEY TRANSPLANT INJURY AND FAILURE
    (2021-05) Vasiļvolfa, Aiga; Kroiča, Juta; Popova, Anna; Rācenis, Kārlis; Šlisere, Baiba; Ziediņa, Ieva; Folkmane, Inese; Lejnieks, Aivars; Čerņevskis, Harijs; Pētersons, Aivars; Kuzema, Viktorija; Rīga Stradiņš University