Browsing by Author "Āboliņš, Arnis"
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Item Krūts vēža molekulāro apakštipu un imūnhistoķīmiskā profila raksturojums. Promocijas darba kopsavilkums(Rīgas Stradiņa universitāte, 2013) Āboliņš, Arnis; Gardovskis, Jānis; Štrumfa, IlzePromocijas darbs “Krūts vēža molekulāro apakštipu un imūnhistoķīmiskā profila raksturojums” veltīts krūts vēža morfoloģiskajai un imūnhistoķīmiskajai izpētei. Krūts vēzis ir viens no biežākajiem ļaundabīgajiem audzējiem Rietumu valstu populācijās un visbiežākais ļaundabīgais audzējs sievietēm. Tā izplatība Latvijā saglabājas augsta. Krūts vēzim raksturīgās heterogenitātes dēļ Pasaules Veselības organizācijas apstiprinātā morfoloģiskā klasifikācija nespēj atklāt visus audzēja parametrus, kas raksturo audzēja bioloģisko potenciālu un ļauj izvēlēties personalizētu terapiju, tādēļ krūts audzēju raksturojumam izmantojama molekulārā klasifikācija. Molekulārie apakštipi sākotnēji tika noteikti, analizējot gēnu ekspresiju ar mikrokartēšanas tehnoloģiju. Šobrīd imūnhistoķīmija tiek pieņemta kā atbilstošākā aizvietojošā tehnoloģija. Promocijas darbā detalizēti raksturoti krūts vēža 5 molekulārie subtipi (lumināls A, lumināls B (HER2 negatīvs), lumināls B (HER2 pozitīvs), HER2 pozitīvs un trīskārši negatīvs) atbilstoši St. Gallēnas klasifikācijai (2011), kas ir novatoriska pieeja arī pasaules praksē, kā arī veikti pētījumi jaunu prognostisku faktoru atklāšanai, analizējot molekulas, kas nosaka audzēja galvenās bioloģiskās īpašības – iesaistās šūnu proliferācijā un šūnu cikla kontrolē (ciklīns D1), ļauj audzēja šūnām izvairīties no apoptozes (BCL2 onkoproteīns), saistīti ar mutācijām proto-onkogēnos (p53) un angioģenēzi (ciklooksigenāze-2). Pētījuma teorētiskās bāzes izstrādei izmantoti 247 literatūras avoti. Promocijas darbā izvirzīts mērķis klasificēt krūts vēža gadījumus pēc molekulāriem apakštipiem un izvērtēt minētos jaunos potenciālos prognostiskos faktorus krūts vēža audos. Izmantojot morfoloģiskas, imūnhistoķīmiskās vizualizācijas un in situ hibridizācijas tehnoloģijas, izpētīti 383 secīgi invazīva krūts vēža gadījumi. Pētījuma rezultātā izveidotas 4 imūnhistoķīmiskās vizualizācijas tehnoloģijas p53 proteīna, BCL2 proteīna, ciklooksigenāzes-2 un ciklīna D1 noteikšanai, detalizēti raksturoti krūts vēža 5 molekulārie apakštipi, iegūts p53, BCL2, ciklooksigenāzes-2 un ciklīnu D1 ekspresējošu krūts vēžu molekulārais portrets un noteikts šo faktoru sastopamības biežums. Kompleksi izvērtējot daudzu parametru prognostisko nozīmi, kā dzīvildzi ietekmējoši faktori identificēti krūts vēža lokālā izplatība (pT) un reģionālo limfmezglu metastātisks bojājums (pN), audzēja diferenciācijas pakāpe, molekulārais subtips, kā arī p53 un BCL2 ekspresija.Item Molecular Subtypes and Immunohistochemical Profiles in Breast Cancer. Doctoral Thesis(Rīga Stradiņš University, 2013) Āboliņš, Arnis; Gardovskis, Jānis; Štrumfa, IlzeThe doctoral thesis “Molecular subtypes and immunohistochemical profiles in breast cancer” is devoted to morphological and immunohistochemical research on breast cancer. Breast cancer is one of the most common malignant tumours in the European population and the most frequent malignancy in female. In Latvia, the incidence of breast cancer remains significant. Breast cancer is a heterogeneous disease including several entities with different clinical behaviour. The classics of breast cancer characteristics are represented in the classification of breast tumours by the World Health Organization. However, even tumours belonging to the same histologic type can have different clinical course. Additional information can be obtained from molecular subtyping of breast cancer thus disclosing subgroups with different biological properties and response to treatment. The molecular subtypes initially were discovered by gene expression profiling in high throughput microarray technologies. At present, immunohistochemistry is accepted as adequate surrogate marker. In the present work, 5 molecular subtypes of breast cancer (luminal A, luminal B (HER2 positive), luminal B (HER2 negative), HER2 positive and triple negative) are detected according to novel St. Gallen (2011) classification and characterised in detail. Further, new potential prognostic factors are analysed, targeting proteins that are involved in the cardinal tumour features as cell proliferation and cell cycle control (cyclin D1), evasion of apoptosis (BCL2 oncoprotein), expression of oncoproteins due to mutations in proto-oncogenes (p53) and angiogenesis (cyclooxygenase-2). The theoretical basis of the doctoral thesis employs 247 literature sources. The aim of the doctoral thesis was to classify breast cancer by molecular subtypes and to evaluate the above listed novel prognostic factors by immunohistochemistry. Within the research work, 383 patients with primary invasive breast cancer were enrolled. The tumour tissues were evaluated by morphological, immunohistochemical visualisation and in situ hybridisation technologies. In the result, 4 immunohistochemical technologies for the detection of p53, BCL2 protein, cyclooxygenase-2 and cyclin D1 have been developed, 5 molecular subtypes of breast cancer are characterised in detail and the molecular portraits of p53, BCL2, cyclooxygenase-2 and cyclin D1-positive breast cancers are obtained. The complex evaluation of the prognostic value of several factors, revealed that the local spread (pT) of breast cancer, regional lymph nodes status (pN) cancer grade and molecular subtype as well as expression of p53 and BCL2 influences the survival.Item Molecular Subtypes and Immunohistochemical Profiles in Breast Cancer. Summary of the Doctoral Thesis(Rīga Stradiņš University, 2013) Āboliņš, Arnis; Gardovskis, Jānis; Štrumfa, IlzeThe doctoral thesis “Molecular subtypes and immunohistochemical profiles in breast cancer” is devoted to morphological and immunohistochemical research on breast cancer. Breast cancer is one of the most common malignant tumours in the European population and the most frequent malignancy in female. In Latvia, the incidence of breast cancer remains significant. Breast cancer is a heterogeneous disease including several entities with different clinical behaviour. The classics of breast cancer characteristics are represented in the classification of breast tumours by the World Health Organization. However, even tumours belonging to the same histologic type can have different clinical course. Additional information can be obtained from molecular subtyping of breast cancer thus disclosing subgroups with different biological properties and response to treatment. The molecular subtypes initially were discovered by gene expression profiling in high throughput microarray technologies. At present, immunohistochemistry is accepted as adequate surrogate marker. In the present work, 5 molecular subtypes of breast cancer (luminal A, luminal B (HER2 positive), luminal B (HER2 negative), HER2 positive and triple negative) are detected according to novel St. Gallen (2011) classification and characterised in detail. Further, new potential prognostic factors are analysed, targeting proteins that are involved in the cardinal tumour features as cell proliferation and cell cycle control (cyclin D1), evasion of apoptosis (BCL2 oncoprotein), expression of oncoproteins due to mutations in proto-oncogenes (p53) and angiogenesis (cyclooxygenase-2). The theoretical basis of the doctoral thesis employs 247 literature sources. The aim of the doctoral thesis was to classify breast cancer by molecular subtypes and to evaluate the above listed novel prognostic factors by immunohistochemistry. Within the research work, 383 patients with primary invasive breast cancer were enrolled. The tumour tissues were evaluated by morphological, immunohistochemical visualisation and in situ hybridisation technologies. In the result, 4 immunohistochemical technologies for the detection of p53, BCL2 protein, cyclooxygenase-2 and cyclin D1 have been developed, 5 molecular subtypes of breast cancer are characterised in detail and the molecular portraits of p53, BCL2, cyclooxygenase-2 and cyclin D1-positive breast cancers are obtained. The complex evaluation of the prognostic value of several factors, revealed that the local spread (pT) of breast cancer, regional lymph nodes status (pN) cancer grade and molecular subtype as well as expression of p53 and BCL2 influences the survival.Item Pathological Complete Remission in Young Colon Cancer Patient with a Large Liver Metastasis after FOLFOX-4/Bevacizumab Treatment – A Case Report(2012-01-01) Skuja, Elīna; Āboliņš, Arnis; Priedīte, Ilze; Purkalne, Gunta; Štrumfa, Ilze; Vilmanis, Jānis; Kalniete, Dagnija; Miklaševičs, Edvīns; Gardovskis, Jānis; Onkoloģijas institūtsItem Torsion of Arteriovenous Malformation - A Rare Caes of Acute Abdomen(2014-10-28) Kokaine, Linda; Lēmanis, Andis; Šapovalovs, Sergejs; Āboliņš, Arnis; Balodis, ArtursArteriovenous malformation (AVM) is a rare finding in gastrointestinal tract usually asymptomatic, although sometimes causes gastrointestinal bleeding or pain. Even more rarely it can be found in other abdominal organs like liver, pancreas, spleen etc. We present a unique case of acute abdominal pain – a patient with torsion of arteriovenous malformation of gastroepiploic arteries with following ischemia and thrombosis causing severe pain. A search at the literature has not yielded any other case report describing similar cause of the acute abdomen.